LOCUS       40924_40442        7208 bp DNA     circular SYN 18-DEC-2018
DEFINITION  Cloning vector pSINTK DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7208)
  AUTHORS   Suemizu H, Ito M.
  TITLE     pSINTK cloning vector for homologous recombination
  JOURNAL   Published Only in Database (2005)
REFERENCE   2  (bases 1 to 7208)
  AUTHORS   Suemizu H, Ito M.
  TITLE     Direct Submission
  JOURNAL   Submitted (17-NOV-2005) Contact:Hiroshi Suemizu Central Institute 
            for Experimental Animals, Biomedical Research Department; 3-25-12 
            Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-0821, Japan
REFERENCE   3  (bases 1 to 7208)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 7208)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Published 
            Only in Database (2005)"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (17-NOV-2005) Contact:Hiroshi Suemizu Central Institute for 
            Experimental Animals, Biomedical Research Department; 3-25-12 
            Tonomachi, Kawasaki-ku, Kawasaki, Kanagawa 210-0821, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
FEATURES             Location/Qualifiers
     source          1..7208
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        36..54
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     misc_feature    72..96
                     /note="multiple cloning site 1 (Sfi I, Not I, Nhe I, Kpn
                     I)"
     protein_bind    148..181
                     /label=loxP
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        378..877
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     CDS             891..1691
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
     misc_feature    1703..1884
                     /label=DNA fragment contains poly A signal
                     /note="DNA fragment contains poly A signal"
     regulatory      1738..1743
                     /label=poly A signal
                     /note="poly A signal"
                     /regulatory_class="polyA_signal_sequence"
     misc_feature    2007..2041
                     /label=lox p site 2
                     /note="lox p site 2"
     protein_bind    complement(2008..2041)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     misc_feature    2055..2088
                     /label=multiple cloning site 2 (Xba I, Eco T22I, Xho I)
                     /note="multiple cloning site 2 (Xba I, Eco T22I, Xho I)"
     regulatory      2089..2596
                     /gene="mouse phosphoglycerate kinase-1"
                     /regulatory_class="promoter"
     gene            2089..2596
                     /gene="mouse phosphoglycerate kinase-1"
                     /label=mouse phosphoglycerate kinase-1
     promoter        2093..2592
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     5'UTR           2634..2673
                     /label=HSV TK 5'-UTR
                     /note="5' untranslated region from the herpes simplex virus
                     thymidine kinase gene"
     CDS             2691..3818
                     /label=HSV TK
                     /note="herpes simplex virus thymidine kinase"
     polyA_signal    3863..3911
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase
                     polyadenylation signal (Cole and Stacy, 1985)"
     promoter        complement(4430..4448)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4458..4474)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     rep_origin      4616..5071
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow
                     indicates direction of (+) strand synthesis"
     promoter        5097..5201
                     /label=AmpR promoter
     CDS             5202..6059
                     /label=AmpR
                     /note="beta-lactamase"
     rep_origin      6233..6821
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    7109..7130
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     promoter        7145..7175
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7183..7199
                     /label=lac operator
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
       61 aagctgggta cggccaatgc ggccgctagc ggtaccgggc cccccctcga ccgccgctaa
      121 ttccgatcat attcaataac ccttaatata acttcgtata atgtatgcta tacgaagtta
      181 ttaggtctga agaggagttt acgtccagcc aagctttaat gcggtagttt atcacagtta
      241 aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat cgtcatcctc
      301 ggcaccgtca ccctggatgc tgtaggcata ggcttggtta tgccggtact gccgggcctc
      361 ttgcggggaa ttctaccggg taggggaggc gcttttccca aggcagtctg gagcatgcgc
      421 tttagcagcc ccgctgggca cttggcgcta cacaagtggc ctctggcctc gcacacattc
      481 cacatccacc ggtaggcgcc aaccggctcc gttctttggt ggccccttcg cgccaccttc
      541 tactcctccc ctagtcagga agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg
      601 acaaatggaa gtagcacgtc tcactagtct cgtgcagatg gacagcaccg ctgagcaatg
      661 gaagcgggta ggcctttggg gcagcggcca atagcagctt tgctccttcg ctttctgggc
      721 tcagaggctg ggaaggggtg ggtccggggg cgggctcagg ggcgggctca ggggcggggc
      781 gggcgcccga aggtcctccg gaggcccggc attctgcacg cttcaaaagc gcacgtctgc
      841 cgcgctgttc tcctcttcct catctccggg cctttcgacc tgcagccaat atgggatcgg
      901 ccattgaaca agatggattg cacgcaggtt ctccggccgc ttgggtggag aggctattcg
      961 gctatgactg ggcacaacag acaatcggct gctctgatgc cgccgtgttc cggctgtcag
     1021 cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc
     1081 aggacgaggc agcgcggcta tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc
     1141 tcgacgttgt cactgaagcg ggaagggact ggctgctatt gggcgaagtg ccggggcagg
     1201 atctcctgtc atctcacctt gctcctgccg agaaagtatc catcatggct gatgcaatgc
     1261 ggcggctgca tacgcttgat ccggctacct gcccattcga ccaccaagcg aaacatcgca
     1321 tcgagcgagc acgtactcgg atggaagccg gtcttgtcga tcaggatgat ctggacgaag
     1381 agcatcaggg gctcgcgcca gccgaactgt tcgccaggct caaggcgcgc atgcccgacg
     1441 gcgatgatct cgtcgtgacc catggcgatg cctgcttgcc gaatatcatg gtggaaaatg
     1501 gccgcttttc tggattcatc gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca
     1561 tagcgttggc tacccgtgat attgctgaag agcttggcgg cgaatgggct gaccgcttcc
     1621 tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat cgccttctat cgccttcttg
     1681 acgagttctt ctgaggggat ccgctgtaag tctgcagaaa ttgatgatct attaaacaat
     1741 aaagatgtcc actaaaatgg aagtttttcc tgtcatactt tgttaagaag ggtgagaaca
     1801 gagtacctac attttgaatg gaaggattgg agctacgggg gtgggggtgg ggtgggatta
     1861 gataaatgcc tgctctttac tgaaggctct ttactattgc tttatgataa tgtttcatag
     1921 ttggatatca taatttaaac aagcaaaacc aaattaaggg ccagctcatt cctcccactc
     1981 atgatctata gatccggaac ccttaatata acttcgtata atgtatgcta tacgaagtta
     2041 ttaggtccct cgactctaga atcccgggta atgcatctcg aggaattcta ccgggtaggg
     2101 gaggcgcttt tcccaaggca gtctggagca tgcgctttag cagccccgct gggcacttgg
     2161 cgctacacaa gtggcctctg gcctcgcaca cattccacat ccaccggtag gcgccaaccg
     2221 gctccgttct ttggtggccc cttcgcgcca ccttctactc ctcccctagt caggaagttc
     2281 ccccccgccc cgcagctcgc gtcgtgcagg acgtgacaaa tggaagtagc acgtctcact
     2341 agtctcgtgc agatggacag caccgctgag caatggaagc gggtaggcct ttggggcagc
     2401 ggccaatagc agctttgctc cttcgctttc tgggctcaga ggctgggaag gggtgggtcc
     2461 gggggcgggc tcaggggcgg gctcaggggc ggggcgggcg cccgaaggtc ctccggaggc
     2521 ccggcattct gcacgcttca aaagcgcacg tctgccgcgc tgttctcctc ttcctcatct
     2581 ccgggccttt cgacctgcag cgacccgctt aacagcgtca acagcgtgcc gcagatcttg
     2641 gtggcgtgaa actcccgcac ctcttcggca agcgccttgt agaagcgcgt atggcttcgt
     2701 acccctgcca tcaacacgcg tctgcgttcg accaggctgc gcgttctcgc ggccatagca
     2761 accgacgtac ggcgttgcgc cctcgccggc agcaagaagc cacggaagtc cgcctggagc
     2821 agaaaatgcc cacgctactg cgggtttata tagacggtcc tcacgggatg gggaaaacca
     2881 ccaccacgca actgctggtg gccctgggtt cgcgcgacga tatcgtctac gtacccgagc
     2941 cgatgactta ctggcaggtg ctgggggctt ccgagacaat cgcgaacatc tacaccacac
     3001 aacaccgcct cgaccagggt gagatatcgg ccggggacgc ggcggtggta atgacaagcg
     3061 cccagataac aatgggcatg ccttatgccg tgaccgacgc cgttctggct cctcatatcg
     3121 ggggggaggc tgggagctca catgccccgc ccccggccct caccctcatc ttcgaccgcc
     3181 atcccatcgc cgccctcctg tgctacccgg ccgcgcgata ccttatgggc agcatgaccc
     3241 cccaggccgt gctggcgttc gtggccctca tcccgccgac cttgcccggc acaaacatcg
     3301 tgttgggggc ccttccggag gacagacaca tcgaccgcct ggccaaacgc cagcgccccg
     3361 gcgagcggct tgacctggct atgctggccg cgattcgccg cgtttacggg ctgcttgcca
     3421 atacggtgcg gtatctgcag ggcggcgggt cgtggcggga ggattgggga cagctttcgg
     3481 ggacggccgt gccgccccag ggtgccgagc cccagagcaa cgcgggccca cgaccccata
     3541 tcggggacac gttatttacc ctgtttcggg cccccgagtt gctggccccc aacggcgacc
     3601 tgtacaacgt gtttgcctgg gccttggacg tcttggccaa acgcctccgt cccatgcacg
     3661 tctttatcct ggattacgac caatcgcccg ccggctgccg ggacgccctg ctgcaactta
     3721 cctccgggat ggtccagacc cacgtcacca cccccggctc cataccgacg atctgcgacc
     3781 tggcgcgcac gtttgcccgg gagatggggg aggctaactg aaacacggaa ggagacaata
     3841 ccggaaggaa cccgcgctat gacggcaata aaaagacaga ataaaacgca cgggtgttgg
     3901 gtcgtttgtt cataaacgcg gggttcggtc ccagggctgg cactctgtcg ataccccacc
     3961 gagaccccat tggggccaat acgcccgcgt ttcttccttt tccccacccc accccccaag
     4021 ttcgggtgaa ggcccagggc tcgcagccaa cgtcggggcg gcaagccctg ccatagccac
     4081 gggccccgtg ggttagggac ggggtccccc atggggaatg gtttatggtt cgtgggggtt
     4141 attattttgg gcgttgcgtg gggtcaggtc cacgactgga ctgagcagac agacccatgg
     4201 tttttggatg gcctgggcat ggaccgcatg tactggcgcg acacgaacac cgggcgtctg
     4261 tggctgccaa acacccccga cccccaaaaa ccaccgcgcg gatttctggc gccgccggac
     4321 gaactaaacc tgactacggc atctctgccc cttcttcgct ggtacgagga gcgcttttgt
     4381 tttgtattgg tcaccacggc cgagtttccg cggtggagct ccaattcgcc ctatagtgag
     4441 tcgtattacg cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc
     4501 gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa
     4561 gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg
     4621 ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca
     4681 cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc
     4741 gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct
     4801 ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg
     4861 ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc
     4921 ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg
     4981 attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg
     5041 aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga aatgtgcgcg
     5101 gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc atgagacaat
     5161 aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt caacatttcc
     5221 gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct cacccagaaa
     5281 cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt tacatcgaac
     5341 tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt tttccaatga
     5401 tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac gccgggcaag
     5461 agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac tcaccagtca
     5521 cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct gccataacca
     5581 tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg aaggagctaa
     5641 ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg gaaccggagc
     5701 tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca atggcaacaa
     5761 cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa caattaatag
     5821 actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt ccggctggct
     5881 ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc attgcagcac
     5941 tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg agtcaggcaa
     6001 ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt aagcattggt
     6061 aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt catttttaat
     6121 ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc ccttaacgtg
     6181 agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct tcttgagatc
     6241 ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg
     6301 tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc ttcagcagag
     6361 cgcagatacc aaatactgtc cttctagtgt agccgtagtt aggccaccac ttcaagaact
     6421 ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct gctgccagtg
     6481 gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat aaggcgcagc
     6541 ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg acctacaccg
     6601 aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa gggagaaagg
     6661 cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg gagcttccag
     6721 ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga cttgagcgtc
     6781 gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct
     6841 ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc
     6901 ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc
     6961 gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac
     7021 cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact
     7081 ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc
     7141 aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat
     7201 ttcacaca
//
